#2720 thermal cycler touchdown pcr manual#
Note: Although manual hotstart PCR can increase specificity and yield. And of course, it provides the same reliability and performance that customers around the world have come to expect from Applied Biosystems thermal cyclers. Thermal cycling parameters Veriti Thermal Cyclers. Thermal cyclers without height-adjustable. If there is no predefined detector for this application, create a new one. Product Description The Applied Biosystems 2720 Thermal Cycler combines industry-standard technology from our GeneAmp PCR System 9700but in a more compact package and at a lower price. Most regular PCR cyclers accept regular profile and high profile file products. Create a new experiment, choosing Absolute Quantification assay type.For more details on the analysis, you can refer to this paper: “The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability.” Nat Protoc 2007 2(9):2212-21.įor an experiment using SDS v1.x, follow the directions below: reLink will attempt to replace product prior to issuing credit.
#2720 thermal cycler touchdown pcr software#
If you have the older software (v 1.x), you will have to program the software differently (see below) and analyze the results independently. Applied Biosystems 2720 PCR Thermal Cycler - OEM: Applied Biosystems. If you have the newer version of the software (v2.0.1 or later), which creates *.eds files, your data will be directly compatible with our Protein Thermal Shift™ Software. Comprehensive service will remain available for both instruments for five years following the discontinuation dates specified in the table below. These products are no longer available for sale.
Gene expression, genotyping, copy number variation, HRM, protein thermal shift, protein detection, mutation detection, miRNA, presence/absence The GeneAmp PCR System 9700, the Applied Biosystems 2720 Thermal Cycler, and the Veriti Thermal Cycler have been discontinued. Tungsten-halogen lamp, 5 excitation filters, 5 emission filters, CCD Cameraįor Research Use Only. FAM™, SYBR®, VIC®, ROX™, NED™, TAMRA™ dyes (Cy®3, Cy®5, and Texas Red® dyes – 7500F only)įast or standard (7500 has standard mode only)
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